Browsing by Subject "Seed germination"

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    Effect of cerium on seed germination and early seedling growth of wheat
    (University Of North Bengal, 2011-03) Shyam, Radhe; Aery, NC
    The effect of various concentrations of three species of cerium was studied on seed germination and early seedling growth of Triticum aestivum L. Seeds were germinated on moist filter papers added with 0.1, 0.5, 2.5, 12.5 and 62.5 Hg g' cerium as cerium chloride, cerium sulphate and cerium nitrate and observations were made on seed germination, shoot- root length, fresh and dry weight. Lower concentrations of cerium species significantly increased shoot-root growth and relative yield of seedlings. Higher concentrations (12.5 and 62.5 Hg g) proved to be toxic for seedling growth. The root growth was affected more adversely than shoots. Though germination speed was affected, ultimate germination was always 100 %. The results of this study indicate that low levels/ concentrations of cerium are beneficial for the test plants.
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    In vivo seed germination and seedling morphology of Phoenix dactylifera L. and Phoenix sylvestris (L) Roxb
    (University of North Bengal, 2016-03) Mondal, Sujit; Chowdhury, Anurag; Basu, S.K; Chowdhury, Monoranjan
    A comparative study of Phoenix dactylitera L. and Phoenix sylvestris (L) Roxb. has been done in the Medicinal Plant Garden in North Bengal University. The germination status of mature seeds of two species in vivo condition was recorded. It was seen that Phoenix dactylifera prefers natural pH (45.5) where as Phoenix sylvestris orefer acidic soil with pH (6-7). During this study total seed output, times of germination, first aerial lea veneration pattem and reproductive capacity were calculated.
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    Mass Propagation of an epiphytic orchid Acampe papillosa (Lindl.) through in vitro seed germination
    (University of North Bengal, 2014-03) Roy, Subhas Chandra
    A method of in vitro propagation of a medicinally important epiphytic orchid of NBU campus Acampe papillosa has been developed. Three different categories of media (1/2 MS, MS and Knudson C) were used for in vitro asymbiotic seed germination for conservation and commercial uses. Seed germination was observed in all the media without any growth supplement or additives although not efficiently. Seeds showed positive germination response in all the three media but the frequency and onset of germination, protocorm formation and associated morphogenic changes leading to plantlets establishment varied with the nature of additional growth stimulus. Different concentrations and combinations of auxin and cytokinins were used to enhance the germination and seedling development. The different concentration of coconut water (CW) was used as additive supplement in this study and 15% CW showed good germination within 22 days in KnC medium. Various concentrations and combinations of plant growth regulators BAP (1mg l-1 to 4 mg l-1), NAA (0.5 mg l-1 to 2 mg l-1) and IBA (0.5 mg l-1 to 2 mg l-1) were added into the three categories of media as growth stimulants. The induction of PLBs was observed in the absence of any exogenous supply of PGRs, but the addition of BAP (4 mg l-1) with KnC medium improved the shoot growth significantly and enhanced the PLBs development (95% efficiency) with highest shoot length (2.5 cm) after six months of culture. Minimum number of days was required for seedling development (148 days) when cultured into the KnC medium supplement with combinations of BAP (4 mg l-1) and IBA (2 mg l-1). Addition of small quantity of NAA (0.5 mg l-1to 2 mg l-1) was not improved the PLBs or seedling development in compared to BAP and IBA. Rooting was improved by addition of IBA (0.5 mg l-1 to 2 mg l-1) into the media. High rate of rooting efficiency was recorded when combinations of auxin and cytokinin was used. Frequency of rooting (95%) was studied in KnC medium containing BAP (4 mg l-1) and IBA (2 mg l-1). Seedling of 2.5 cm long with 3-4 leaves were transferred to earthen pot containing wood charcoal and coco peat for acclimatization under green house conditions. Hardened plantlets showed significantly high survival rate (81%) after seven months of transfer. Therefore, the present method of micro-propagation could be used successfully to propagate this important orchid for commercial production and conservation purposes.
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    Synthesis, characterization and applications of some nanoparticles supplemented with living and chemical substances
    (University of North Bengal, 2022) Das, Debasmita; Roy, Mahendra Nath