NBU Journal of Plant Sciences
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The NBU Journal of Plant Sciences serves as the official organ of the Department of Botany for the publication of research papers by its members and researchers. The journal is sent free to its members and different academic institutions on demand.
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Item Open Access Association of AM fungi in the rhizosphere of Thuja orientalis (L)(University of North Bengal, 2013-03) De, U.K.; Das Biswas, R.; Chakraborty, B.N.Thuja orientalis (L) is used as an ornamental plant as well as medicinal plant in India from prehistoric times and instances were found in various manuscripts revealing its medicinal property against abdominal pain, hook worms, arthritis etc. AMF associated with rhizosphere of Thuja were extensively studied in relation to their population in soil, root-length colonization, histopathological study as well as their diversification. Predominant existence of various species of Glomus, Gigaspora were determined and their spore surface texture was examined using stereo microscope, besides, species of Acaulospora, Scutellospora etc. were also documented. Histopathological studies of host roots showed various types of hyphal network and arbuscules, AMF were tried to identify up to species level with the help of standard keys. The result indicated that the various spp. of AMF have established their colonization in host roots and the host plant have a significant role in root tissue colonization.Item Open Access Root colonization of mandarin plants grown in orchards of Darjeeling hills and plains with arbuscular mycorrhizal fungi and their effects on plant growth(University of North Bengal, 2012-03) Allay, S.; De, U.K.; Chakraborty, B.N.Citrus reticulata is an ancient commercial crop being cultivated in Darjeeling-Sikkim hills. Many diseases are prevalent in mandarin plants, out of them, bacterial & fungal diseases are mostly dangerous. Arbuscular Mycorrhizal Fungi were screened from rhizosphere of mandarin plants from the four different regions using wet sieving and decanting method. Microscopical observation revealed the presence of different genus of AM fungi present in the root as hyphae, spores and sporocarp. Glomus mosseae, G. fasciculatum, G. aggregatum, G. badium. G. constrictum, G. versiforme, Gigaspora gigantea, G. margarita, Acaulospora capsicula, A. bireticulata, Sclerocystis and Scutellospora rubra were found to be dominant in all the soil samples of mandarin. Species of Glomus were found to be high in both hilly and foothill regions. Glomus mosseae and G. fasciculatum were selected for mass multiplication in maize plant in pots. Histopathological study of root showed the presence of vesicles and arbuscules. AMF infection and total number of spores per 100 gram of soil were recorded. Scanning Electron Microscopy (SEM) of AMF spores of mandarin revealed clear morphology, spore wall characters and hyphal attachment of spores. Total phosphate content of the soil, soil analysis and enzyme activities in roots and leaves of mandarin plant from the different regions were studied. Three major defense enzymes peroxidase, chitinase and β- 1,3- glucanase showed enhanced activities and the total phosphate content also decreased in soil with respect to control. Present study evaluates the effect of AMF in plant growth and phosphate solubilization.