NBU Journal of Plant Sciences

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The NBU Journal of Plant Sciences serves as the official organ of the Department of Botany for the publication of research papers by its members and researchers. The journal is sent free to its members and different academic institutions on demand.

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2015 - 201912020 - 20221

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Subject: search.filters.subject.Phytopathogen

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    ItemOpen Access
    Antifungal Efficacy of Cu-Based Nano-Chitosan on Rhizopus stolonifer, A Virulent Phytopathogen
    (University of North Bengal, 2022) Chouhan, Divya; Choudhuri, Chandrani; Dutta, Poulami; Mandal, Palash; Mathur, Piyush
    Agro-scientists are giving endless efforts for synthesizing a bio-derived molecule that can act as a promising antifungal agent for combating a large number of phytopathogens. Harmful phytopathogens decrease crop yield and its quality. Rhizopus stolonifer is one such virulent phytopathogen that causes huge losses during the post-harvest period of crops. This pathogen mainly causes rot disease in fruits, crops, and vegetables. The second most abundantly available biological macromolecule, Chitosan and its metal-based nanoparticles stands as a potential antifungal agent for combating Rhizopus stolonifer. This study includes the synthesis of Cu chitosan nanoparticles (Cu-CNPs) and chitosan nanoparticles (CNPs) through the ionic gelation method and its characterization based on UV Vis spectrophotometer, FE-SEM, EDXS, and DLS. Cu-CNPs and CNPs were screened from 100-2000 µg/mL concentration against R. stolonifer for the assessment of its antifungal activity. Spore viability assay and lipid peroxidation of the pathogen using Cu-CNPs and CNPs were also determined. Generation of oxidative stress in the mycelium of the pathogen on the application of Cu-CNPs and CNPs was traced by fluorescence microscopy. Changes in the ultra-structure of the sporangium of R. stolonifer after treatment with Cu-CNPs and CNPs were visualized under SEM. Results showed that Cu-CNPs inhibit the growth of R. stolonifer at 2000 µg/mL and elevate malonaldehyde (MDA) content in the pathogen as a result of lipid peroxidation and produces defined damages on the sporangium membrane as observed under electron microscope. Fluorescence microscopy revealed the emission of high intensity of fluorescence due to the generation of oxidative stress in Cu-CNPs treated fungal mycelium.
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    ItemOpen Access
    Screening of free-living bacteria from the rhizosphere of Jute for their multiple plant growth promoting and antagonistic activity against phytopathogens
    (University of North Bengal, 2015-03) Majumdar, Sukanta; Chakraborty, Usha
    Present investigation was undertaken to screen the PGPR isolates from the rhizosphere of Jute for their plant growth promoting and antagonistic activities in the view of an alternative way to chemical fertilizer and hazardous fungicides. A total 76 isolates were isolated from different parts of northern West Bengal and screened for their antagonistic activity against Macrophomiona phaseolina. Most promising five isolates were selected for further study and screened for other plant growth promoting and lytic enzyme producing abilities. Out of these, two isolates were Gram positive and rest three Gram negative. All five isolates exhibited several plant growth promoting activities. All five isolates showed IAA and ammonia production whereas four out five showed phosphate solubilization activity. Three PGPR strains exhibited siderophore production and only one isolate showed cyanide production ability. Among the lytic enzymes, chitinase was produced by three isolates. Among them B-3 showed highest degree of chitinase production. Protease was also produced by four strains but amylase and B-1,4-glucanase activity showed by only one isolate, Act-6. Two isolates B-3 and Acti-6 showed considerable amount of antagonistic activity against three phytopathogens Macrophomina phaseolina, Fusarium oysporum, and F. semitectum suggesting that Acti-6 and B-3 showed several attributes to be the potent strains of PGPR and can be used as biofertilizer as well as biocontrol agents.