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Item Open Access Antifungal Efficacy of Cu-Based Nano-Chitosan on Rhizopus stolonifer, A Virulent Phytopathogen(University of North Bengal, 2022) Chouhan, Divya; Choudhuri, Chandrani; Dutta, Poulami; Mandal, Palash; Mathur, PiyushAgro-scientists are giving endless efforts for synthesizing a bio-derived molecule that can act as a promising antifungal agent for combating a large number of phytopathogens. Harmful phytopathogens decrease crop yield and its quality. Rhizopus stolonifer is one such virulent phytopathogen that causes huge losses during the post-harvest period of crops. This pathogen mainly causes rot disease in fruits, crops, and vegetables. The second most abundantly available biological macromolecule, Chitosan and its metal-based nanoparticles stands as a potential antifungal agent for combating Rhizopus stolonifer. This study includes the synthesis of Cu chitosan nanoparticles (Cu-CNPs) and chitosan nanoparticles (CNPs) through the ionic gelation method and its characterization based on UV Vis spectrophotometer, FE-SEM, EDXS, and DLS. Cu-CNPs and CNPs were screened from 100-2000 µg/mL concentration against R. stolonifer for the assessment of its antifungal activity. Spore viability assay and lipid peroxidation of the pathogen using Cu-CNPs and CNPs were also determined. Generation of oxidative stress in the mycelium of the pathogen on the application of Cu-CNPs and CNPs was traced by fluorescence microscopy. Changes in the ultra-structure of the sporangium of R. stolonifer after treatment with Cu-CNPs and CNPs were visualized under SEM. Results showed that Cu-CNPs inhibit the growth of R. stolonifer at 2000 µg/mL and elevate malonaldehyde (MDA) content in the pathogen as a result of lipid peroxidation and produces defined damages on the sporangium membrane as observed under electron microscope. Fluorescence microscopy revealed the emission of high intensity of fluorescence due to the generation of oxidative stress in Cu-CNPs treated fungal mycelium.Item Open Access Assessment of Growth Performance and Histochemical Localisation of Reactive Oxygen Species in Fenugreek under Exogenous Calcium Ion Priming(University of North Bengal, 2022) Gupta, Saran Kumar; Mandal, Palashobjective of present study was to evaluate the response of the fenugreek seeds primed with various elicitors such as calcium chloride (CC) as an exogenous source of calcium ion; a calcium chelator: Ethylene glycol-bis(2-aminoethylether)-N,N,N´,N, tetra acetic acid (EG); and Lanthanum chloride (LC): a calcium channel blocker, under salinity stress. Significant improvement in the growth parameters of fenugreek seedlings was observed comparing with control. The stress tolerance index (STI) and histochemical detection of reactive oxygen species were performed to evaluate the tolerance of the fenugreek against salinity stress. The results exhibited noteworthy inhibitory effect of salinity stress in control set which was significantly mitigated by the exogenous calcium ion application. Furthermore, under the influence of calcium ion antagonists, EG and LC the adverse effect of salinity was more prominent than control set. In conclusion present investigation revealed that exogenous calcium ion is an ideal elicitor for enhancing growth and development of the fenugreek with better salinity stress management.Item Open Access Study of Major Isoflavones in Mungbean Seedlings with Special Emphasis on Its Enhanced Antioxidant Activity After Solid Matrix Priming with Selected Elicitors Including Nano-Chitosan Under Salinity Stress(University of North Bengal, 2022) Sen, Sujoy Kumar; Mandal, Palash; Bhandari, Jnan BikashFor a long time, mung bean has been a well-liked crop. It is frequently used as a popular dish in the primarily cereal-based diets of Asian countries for its physiological functionalities, such as antioxidant, antitumor, and antidiabetic activities. Isoflavones present in legume-based foods have high antioxidant potential. These isoflavones are considered beneficial to human health and are linked to a reduced risk of cardiovascular disease, osteoporosis, and the prevention of certain types of cancer in humans, including breast, prostate, and colon cancer, as well as menopausal symptoms. On the other hand, nanotechnology is starting to look like an excellent method to boost food production and make farming less hazardous to the environment. Fascinatingly, the seed nano-priming method demonstrated promising results to mitigate the detrimental effects of different abiotic stress factors including salinity stress on crop plants and has thus, led to higher crop yields. The current study aimed to evaluate the effects of solid matrix priming (SMP) using nano-chitosan in mung bean sprouts under salinity stress related to the production of major mung bean isoflavones, which were detected through high-resolution liquid chromatography-mass spectrometry. When compared to unprimed seedlings exposed to salinity stress conditions, phytochemical quantification showed that SMP with nano-chitosan showed improved antioxidant activities as well as the highest total flavonoids and proline content. Under salinity stress, SMP with nano-chitosan significantly increased the biochemical anti-oxidative properties in germinated mung bean seeds, and also provided salt tolerance. As a familiar healthier choice, and because of the significance of mung bean sprouts for human health and the industry's rapid expansion, nutritional enrichment of this food has emerged as a significant field of study.Item Open Access Zinc Oxide Nanoparticles: Different synthesis approaches and applications(University of North Bengal, 2021-03) Kundu, Sudipta; Haydar, Md. Salman; Mandal, PalashNanotechnology deals with the synthesis and usage of materials with nanoscale dimension (1-100 nm). Nanoscale dimensions of the particles provide large surface to volume ratio and thus very specific properties. Synthesis of zinc oxide nanoparticles (ZnO NPs) has gained prime importance in recent arena due to its high excitation binding energy and large bandwidth and it has potential applications like anti-diabetic, antibacterial, anti-inflammatory, antifungal, wound healing, antioxidant and optical properties. Zinc (Zn) is a common mineral element in nature which plays an immense role in many biological processes. It is defined as an essential trace element or micronutrient which is very much crucial for the normal growth and the development of all higher plants as well as animals. Zinc directly involves in enzyme function associated with the photosynthesis and energy process in plants. It also plays an important role in maintenance of membrane integrity, formation and production of growth hormone, insulin, thyroid etc. Due to the involvement of large rate of toxic chemicals and requirement of extreme environment, chemical and physical methods of nanoparticle synthesis often became inappropriate. Whereas, green methods are used in a wide range of biological samples including plants, fungus, bacteria, and algae, which act as both reducing and capping agent. Biologically synthesized zinc nanoparticles have been reported for versatile applications in the field of medicine and pharmacy, for bio-imaging and bio-sensor production, in gene therapy and drug delivery system. Zinc nanoparticles also play vital role in agricultural sector including plant growth and development, enhancement of crop yield and post-harvest processing. In spite of being great potential of ZnO NPs for abiotic and biotic stress management, research works in this field is considerably less. This review described the summary of the recent works in the synthesis mechanism, characterization techniques, and applications of biosynthesized ZnO NPs in medicine and agriculture with special reference to application on plant growth, development and abiotic stress management.