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    Assessment of Growth Performance and Histochemical Localisation of Reactive Oxygen Species in Fenugreek under Exogenous Calcium Ion Priming
    (University of North Bengal, 2022) Gupta, Saran Kumar; Mandal, Palash
    objective of present study was to evaluate the response of the fenugreek seeds primed with various elicitors such as calcium chloride (CC) as an exogenous source of calcium ion; a calcium chelator: Ethylene glycol-bis(2-aminoethylether)-N,N,N´,N, tetra acetic acid (EG); and Lanthanum chloride (LC): a calcium channel blocker, under salinity stress. Significant improvement in the growth parameters of fenugreek seedlings was observed comparing with control. The stress tolerance index (STI) and histochemical detection of reactive oxygen species were performed to evaluate the tolerance of the fenugreek against salinity stress. The results exhibited noteworthy inhibitory effect of salinity stress in control set which was significantly mitigated by the exogenous calcium ion application. Furthermore, under the influence of calcium ion antagonists, EG and LC the adverse effect of salinity was more prominent than control set. In conclusion present investigation revealed that exogenous calcium ion is an ideal elicitor for enhancing growth and development of the fenugreek with better salinity stress management.
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    ItemOpen Access
    Study of Major Isoflavones in Mungbean Seedlings with Special Emphasis on Its Enhanced Antioxidant Activity After Solid Matrix Priming with Selected Elicitors Including Nano-Chitosan Under Salinity Stress
    (University of North Bengal, 2022) Sen, Sujoy Kumar; Mandal, Palash; Bhandari, Jnan Bikash
    For a long time, mung bean has been a well-liked crop. It is frequently used as a popular dish in the primarily cereal-based diets of Asian countries for its physiological functionalities, such as antioxidant, antitumor, and antidiabetic activities. Isoflavones present in legume-based foods have high antioxidant potential. These isoflavones are considered beneficial to human health and are linked to a reduced risk of cardiovascular disease, osteoporosis, and the prevention of certain types of cancer in humans, including breast, prostate, and colon cancer, as well as menopausal symptoms. On the other hand, nanotechnology is starting to look like an excellent method to boost food production and make farming less hazardous to the environment. Fascinatingly, the seed nano-priming method demonstrated promising results to mitigate the detrimental effects of different abiotic stress factors including salinity stress on crop plants and has thus, led to higher crop yields. The current study aimed to evaluate the effects of solid matrix priming (SMP) using nano-chitosan in mung bean sprouts under salinity stress related to the production of major mung bean isoflavones, which were detected through high-resolution liquid chromatography-mass spectrometry. When compared to unprimed seedlings exposed to salinity stress conditions, phytochemical quantification showed that SMP with nano-chitosan showed improved antioxidant activities as well as the highest total flavonoids and proline content. Under salinity stress, SMP with nano-chitosan significantly increased the biochemical anti-oxidative properties in germinated mung bean seeds, and also provided salt tolerance. As a familiar healthier choice, and because of the significance of mung bean sprouts for human health and the industry's rapid expansion, nutritional enrichment of this food has emerged as a significant field of study.