Department of Botany

Permanent URI for this communityhttps://ir.nbu.ac.in/handle/123456789/4383

Since establishment in 1984, the department flourished with tireless efforts of faculties, staff and students. The course curriculum of the department has always been strong and forward looking. Syllabi have been revised regularly and the latest version was done with the introduction of semester system offering five special papers, namely Cytogenetics, Microbiology, Molecular Plant pathology and Fungal Biotechnology, Plant Biochemistry and Taxonomy of Angiosperms and Biosystematics.

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Author: search.filters.author.Saha, A.Subject: search.filters.subject.2-ABA

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    Isolation and identification of a virulent Ralstonia solanacearum by fliC gene amplification and induction of chitinase by 2-amino butyric acid for control of bacterial wilt in tomato plants
    (University of North Bengal, 2013-03) Saha, A.; Mandal, H.; Saha, D.
    Ralstonia solanacearum is a devastating, soil borne bacterial pathogen of tomato. The pathogen is nonmotile in planta but highly motile in culture. On the basis of physiological and biochemical characteristics 26 isolates have been purified and identified as Ralstonia solanacearum. The flic gene is responsible for the movement of bacteria. Ralstonia specific fliC gene amplification is the indication of virulence of the pathogen. In the present study one R. solanacearum isolate has been identified by PCR amplification of the fliC gene using fliC gene specific primer. Following isolation and identification of the virulent isolate, fresh tomato plants were induced by application of 2- amino butyric acid (ABA). The defense enzyme, chitinase was estimated in treated plants. Treated inoculated plants did not show any visible symptoms of wilt even after 14 days of inoculation. Significantly it was observed that chitinase was increased in the 2-ABA-treated plants and also in the treated-inoculated plants. The increased chitinase activity in the treated plants showed that 2-ABA has the resistance inducing capacity in tomato plants against Ralstonia solanacearum.